The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli.
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چکیده
38 Lehman, I. R., G. G. Roussos, and A. Pratt, J. Biol. Chem., in press. 39 Lowry, O., J. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem., 193, 265 (1951). 40 Monod, J., Ann. Inst. Pasteur, 79, 390 (1950). 4] Wiesmeyer, H., and M. Cohn, Biochim. Biophys. Acta, 39, 417 (1960). 42 Lineweaver, H., and D. Burk, J. Am. Chem. Soc., 56, 658 (1934). 43 Doty, P., these PROCEEDINGS, 42, 791 (1956). 44 Markham, R. and J.' P. Smith, Biochem. J., 52, 552 (1952). 4 Magasanik, B., E. Vischer, R. Doniger, D. Elson, and E. Chargaff, J. Biol. Chem., 186, 37 (1950). 46 Weiss, S. B., and T. Nakamoto, these PROCEEDINGS, 47, 1400 (1961). 47 Geiduschek, E. P,., T. Nakamoto, and S. B. Weiss, these PROCEEDINGS, 47, 1405 (1961). 48 Hall, B. D., and S. Spiegelman, these PROCEEDINGS, 47, 137 (1961). 49 Sinsheimer, R. L., J. Mol. Biol., 1, 43 (1959). 60 Rich, A., these PROCEEDINGS, 46, 1044 (1960). 51 Schildkraut, C. L., J. Marmur, J. R. Fresco, and P. Doty, J. Biol. Chem., 236, PC 2 (1961). 62 Wyatt, G. R., and S. S. Cohen, Biochem. J., 55, 774 (1953).
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 48 شماره
صفحات -
تاریخ انتشار 1962